Elucidation of inhibitory mechanisms of bovine lactoferrin for tumor-induced angiogenesis.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Yoshiharu Takayama 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門 畜産物研究領域, 上級研究員 (00343989)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: タンパク質 / 農林水産物 / 細胞・組織 / 受容体 / ラクトフェリン

Outline of Final Research Achievements

To identify the region of bovine lactoferrin that is responsible for suppression of tumor-induced angiogenesis, we compared the effect of lactoferrin fragments on VEGF-induced angiogenesis. C-lobe fragment of bovine lactoferrin was more potent inhibitor for angiogenesis. Bovine lactoferrin interacted with CXCR4-containing lipoparticle. Lactoferrin stimulation mimicked many aspects of SDF-1-induced CXCR4 modification, including receptor dimerization, tyrosine phosphorylation, and ubiquitination, suggesting that CXCR4 acts as a lactoferrin receptor.

Free Research Field

生化学・細胞生物学