2015 Fiscal Year Final Research Report
M13 phage as a vaccine vehcle
| Project/Area Number |
25450427
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ワクチン / バクテリオファージ / ファージディスプレイ / アジュバント / 抗体 / ペプチド |
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| Outline of Final Research Achievements |
M13 phage stimulate an innate immune response and induce a strong primary IgG response in mice without any inflammatory adjuvant materials. To investigate the efficacy of M13 phage as a vaccine carrier for peptide antigens, the sequences of 1-15 region of Aβ or a B-cell epitope of Cry j 1 were genetically linked to the N terminous of M13 gene 3 protein (g3p) or gene 8 protein (g8p). When C57BL/6 mice were immunized subcutaneously with M13 phage displaying the sequences of 1-15 region of Aβ on their g3p molecules, these mice showed a production of serum IgG against Aβ42 in two weeks after the secondary immunization. In the case of recombinant phage contains approximately 300 recombinant g8p molecules, serum IgG against Aβ42 was induced during a primary response. Similar response profiles were observed in M13 phage displaying the sequences of a B-cell epitope of Cry j 1, indicating that there are different immunological mechanisms of phage vaccine between g3p fusion and g8p fusion.
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| Free Research Field |
免疫学
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