Analysis of mechanism of gene expression regulation by testis-specific homeodomain protein

2015 Fiscal Year Final Research Report

• Project/Area Number: 25450465
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: Kagoshima University (2015); Tottori University (2013-2014)
• Principal Investigator: ASANO Atsushi 鹿児島大学, 農水産獣医学域獣医学系, 教授 (90312404)
• Co-Investigator(Kenkyū-buntansha): YAMANO Yoshiaki 鳥取大学, 農学部, 教授 (00182593) ; TAKEUCHI Takashi 鳥取大学, 農学部, 教授 (10325061)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 精子形成 / ホメオドメインタンパク質 / 転写調節

Outline of Final Research Achievements

To clarify the role of transcription factors in the control of gene expression in spermatogenesis, we analyzed expression and function of a homeodomain protein, Nkx2-6, that was estimated to be a testis-specific expressed protein. We found that Nkx2-6 was mainly expressed in testis, and that spermatids highly expressed this protein. In addition, we found that Nkx2-6 had potential as a gene-expression regulator of testis-specific genes, Prm1 and Akap3. In this study, we show part of the function of Nkx2-6 in regulation gene expression associated with spermatogenesis.

Free Research Field

実験動物学