Suppression of the splicing mutation based on the molecular mechanism of pre-mRNA splicing fidelity

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: MAITA Hiroshi 北海道大学, 薬学研究院, 講師 (60431318)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: スプライシング / ケミカルバイオロジー

Outline of Final Research Achievements

We have developed an assay to find a compound that affect the spliceosome and have obtained several hit compounds by the automated screening of a small compound library. In this study we made a genetic reporter to detect the compound-mediated recovery of defected splicing that is caused by a mutation in the 3’ splice site. Some of our hit compounds have shown that they can induce splicing of the mutated intron at the correct site.

Free Research Field

分子生物学