Regulation of cell division by Src signaling and its role in the cancer malignancy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460076
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyoto Pharmaceutical University
• Principal Investigator: Nakayama Yuji 京都薬科大学, 薬学部, 教授 (10280918)
• Co-Investigator(Kenkyū-buntansha): SAITO YOUHEI 京都薬科大学, 薬学部, 助教 (90411032)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Src / 細胞分裂 / チロシンリン酸化 / 紡錘体形成 / v-Src

Outline of Final Research Achievements

To elucidate the mechanisms underlying Src-signaling-mediated regulation of cell division, we demonstrated the involvement of Src tyrosine kinases and their downstream signaling molecules in cell division including spindle formation and chromosome segregation. Furthermore, we established the novel method to synchronize M-phase cells at the anaphase and telophase, and we identified a lot of novel, phosphorylated peptides at this phase. Additionally, by using v-Src as a model protein of aberrantly activated Src, we found that aberrant activation of Src kinase activity induces defect in cell division. We also found the chromosome bridge formation by v-Src possibly through v-Src-induced DNA damage.

Free Research Field

細胞生物学，生化学