Establishment of a new quality assessment method of Kampo based on multilateral analysis.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Okayama University
• Principal Investigator: Shoko TANIGUCHI 岡山大学, 医歯(薬)学総合研究科, 准教授 (20243488)
• Co-Investigator(Kenkyū-buntansha): HATANO Tsutomu 岡山大学, 大学院医歯薬学総合研究科, 教授 (50127578)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 漢方処方 / ポリフェノール / 相互作用

Outline of Final Research Achievements

Constituents in decoctions of a representative Kampo prescription and related formulations were analyzed quantitatively. HPLC conditions for simultaneous analyses of those constituents with a wide range of polarities including hydrolysable tannins were established. Particularly, we focused on the interactions between the constituents affecting the compositional changes in the constituents. Precipitations in the decoctions were also analyzed using UV-visible spectral analyses. Participation of the electrostatic and hydrophobic interactions in forming the precipitations were suggested. We found that GPC analyses are effective for the behaviors of polymeric polyphenols of rhubarb, a representative crude drug, and precipitations are formed as a result of interactions between those polyphenols of rhubarb and constituents derived from some other crude drugs in respective Kampo prescriptions.

Free Research Field

生薬化学