Screening for new antifungal antibiotics of microbial origin using silkworm infection model with Candida albicans

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Kitasato University
• Principal Investigator: Uchida Ryuji 北里大学, 薬学部, 講師 (60280632)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 抗真菌剤 / 感染症 / カイコ評価系 / 微生物資源 / 天然有機化合物 / カンジダ

Outline of Final Research Achievements

The in vivo-mimic assay system using silkworm larvae was performed as the primary screening tool to discover antifungal antibiotics against Candida albicans. 9,500 Microbial cultures were screened in this in vivo-mimic assay system, resulting that five known compounds showing a therapeutic effect were identified. At the same time, in the course of screening for amphotericin B potentiators of microbial origins, four new compounds, designated shodoamides A to D, were isolated from the culture broth of unidentified fungal strain BF-0158. Furthermore, stereochemistries, antifungal activities and mode of action of new antifungal antibiotics, simplifungin and valsafungins A and B, were also elucidated.

Free Research Field

天然物化学