Development of anti-cancer antibodies through high-throughput sequencing from antibody phage library

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460151
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Kagoshima University
• Principal Investigator: ITO YUJI 鹿児島大学, 理工学研究域理学系, 教授 (60223195)
• Co-Investigator(Kenkyū-buntansha): ARIMA Naomichi 鹿児島大学, 医歯学総合研究科, 客員研究員 (30175997)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 抗体医薬 / ガン / ファージライブラリー / 次世代シークエンサー / バイオパンイング / 単鎖Fv抗体 / ヒト抗体

Outline of Final Research Achievements

In this study, we aimed to establish the short-term and reliable system for isolating anti-cancer antibodies (single chain Fv antibodies) from antibody phage library, especially by combining the biopanning and the comprehensive sequence analysis on next generation sequencer (NGS method). As a result, we successfully isolated several clones of lutheran and antigen A-specific antibodies which targeted hepatic and Esophageal cancers, respectively. These results indicate that NGS method is more useful in isolation of multiple antigen-specific antibodies, as compared with the conventional biopanning method comprising of biopanning and cloning/binding screening processes.

Free Research Field

タンパク質工学、免疫学