2015 Fiscal Year Final Research Report
Regulatory mechanisms of estrogen receptor gene expression by alternative promoter usage and alternative splicing
| Project/Area Number |
25460319
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | エストロゲン / エストロゲン受容体 / 多重プロモーター / 選択的スプライシング / スプライス変異体 / 恒常的転写活性化 / 生殖生理学 / 神経内分泌学 |
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| Outline of Final Research Achievements |
To determine regulatory mechanisms of estrogen receptor gene expression, alternative promoter usage and alternative splicing profiles of the estrogen receptor genes were examined. We identified modular structures of the estrogen receptor genes and several splice variants encoding N- and C-terminally-truncated estrogen receptor proteins. Some C-terminally-truncated estrogen receptor variants exhibited constitutive transcriptional transactivation in transfected cells. Ligand-independent transcriptional transactivation by steroid hormone receptors is deduced to be one of the candidate causes of malignant development of hormone-sensitive tumors. Therefore, our results may provide key mechanistic information explaining the cause of hormone-independent cell overgrowth in estrogen-sensitive tumors.
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| Free Research Field |
生殖生理学
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