Analysis of the transcription factor network during the early stage of iPSC production

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460354
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: Hisatake Koji 筑波大学, 医学医療系, 教授 (70271236)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA KEN 筑波大学, 医学医療系, 助教 (80500610)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 転写因子 / 遺伝子発現制御 / リプログラミング

Outline of Final Research Achievements

Genome-wide expression analyses were used to identify transcription factors whose expression decrease at the early stage or reprogramming. They were further narrowed down by other data base to select for the genes that are likely to be regulated directly by Oct4 and Klf4. The selection resulted in 38 candidate genes whose expression changes are potentially important for the early stage of reprogramming. siRNA-mediated knockdown and over-expression of these genes showed that down-regulation of Osr1、Ebf1、Ebf3、Meox2、Smardc3 and Prrx is essential at the early stage of reprogramming. The involvement of these genes in EMT or MET was examined by the used of NMuMG cells, and the Ebf3 was found to have an activity in promoting EMT.

Free Research Field

分子生物学