Maintenance of intestinal homeostasis by enteric neuron and mucosal immune system

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Chiba University
• Principal Investigator: Hatano Masahiko 千葉大学, 医学(系)研究科(研究院), 教授 (20208523)
• Co-Investigator(Kenkyū-buntansha): FUJUMURA Lisa 千葉大学, バイオメディカル研究センター, 助教 (30376363)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 腸管神経系 / 腸管免疫系 / 腸管上皮バリア / 腸内フローラ

Outline of Final Research Achievements

Ncx-deficient (KO) mice exhibited increased number of NO producing enteric neurons. We characterized the fecal microbiota to examine whether increased number of enteric neurons affects enteric flora. CFUs of aerobic bacteria increased 10 to 100-folds in the KO mice compared to those of wild type mice.Since bacterial NO reductase gene V (NorV) which detoxificate NO, determine a virulence of bacteria, we further examined frequency of NorV+ bacteria in feces. More NorV+ bacteria were detected from the feces of the KO mice compared to that of wild type mice.To elucidate whether the dysbiosis affect the severity of the DSS-induced colitis, fecal transfer experiments were carried out. C57BL/6 mice were given either wild type or the KO mice derived feces and challenged with DSS. Mice transferred with feces of the KO mice developed more severe colitis than wild type transferred one. Our study implies that NO derived from EN participates in regulation of intestinalhomeostasis.

Free Research Field

分子生物学、発生工学