KLF14 biology: its physiological roles and transcriptional regulation

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460409
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human genetics
• Research Institution: National Center for Child Health and Development
• Principal Investigator: NAKABAYASHI Kazuhiko 国立研究開発法人国立成育医療研究センター, 周産期病態研究部, 室長 (10415557)
• Co-Investigator(Renkei-kenkyūsha): TAKADA Shuji 国立成育医療研究センター, 研究所, 部長 (20382856) ; TOMIKAWA Junko 国立成育医療研究センター, 研究所, 研究員 (80534990) ; TAYAMA Chiharu 国立成育医療研究センター, 研究所, 研究員 (60774445)
• Research Collaborator: SUGAHARA Naoko 国立成育医療研究センター, 研究所, 研究補助員
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: ゲノムインプリンティング / KLF14 / 白色脂肪組織

Outline of Final Research Achievements

KLF14 has been proposed to act as a master regulator of key metabolic genes in adipose tissue. Cis-regulatory SNPs in the KLF14 locus are associated with susceptibility to multiple metabolic diseases including T2D. We aimed to elucidate physiological functions of KLF14 in the white adipose tissue (WAT), and to establish materials and methods to study molecular mechanisms underlying imprinted gene expression of Mest and Klf14 genes, which are located within a 200kb genomic interval.
We revealed that KLF14 involves in controlling obesity-induced inflammation in WAT by analyzing the phenotype of Klf14 knockout mice. We also successfully established mouse lines with a targeted deletion of the germline differentially methylated region at the Mest promoter region, which is expected to play a central role in the regulation of imprinted expression of Klf14.

Free Research Field

ゲノム科学