2015 Fiscal Year Annual Research Report
大腸鋸歯状病変の発癌過程における分子病理学的制御の解明と鑑別診断への応用
| Project/Area Number |
25460416
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
仙谷 和弘 広島大学, 医歯薬保健学研究院(医), 講師 (30508164)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | Fukutin / SPC18 / BST2 |
|---|
| Outline of Annual Research Achievements |
最終年度である本年度は、CAST法で見出したBone Marrow Stromal Antigen (BST2)の発現が大腸癌のみならず、胃癌や食道癌でも同様に予後不良因子となる原因について生物学的機能解析を行い、EGFR経路の活性化を通じて増殖能を亢進させていることを明らかにした。また、分泌蛋白であるsignal peptidase complex 18 (SPC18)は大腸腫瘍において、特に古典的発癌経路の大腸癌および前癌病変で高発現し、鋸歯状病変の発癌経路に関与するとされるMSI-H大腸癌やSSA/P、TSAなどでは発現はほとんど認められず、発癌メカニズムの違いを明らかにする重要な分子である可能性が示唆された。
更に、2種類の胃癌細胞株を用いたCAST法の比較で見出した福山型筋ジストロフィーの原因遺伝子の一つとして知られるFukutin (FKTN)が胃癌でも43%に発現し、T grade、N gradeの高いもの、分化度の高い組織型と有意な相関が見られることを明らかにした。更に粘液形質との比較ではCD10と有意な相関を示し、腸型粘液形質で頻度が高い傾向が見られた。FKTN-siRNAによるノックダウンによって細胞増殖能が抑制されたが、浸潤能には変化は見られなかった。EGFR経路の分子(EGFR, Akt, ERK1/2, p38およびそのリン酸化)の発現も検討したが有意な変化は認められなかった。またFukutinの発現は予後に有意な影響は及ぼさなかった。
|
-
[Journal Article] Mouse model of proximal colon-specific tumorigenesis driven by microsatellite instability-induced Cre-mediated inactivation of Apc and activation of Kras.2016
Author(s)
Kawaguchi Y, Hinoi T, Saito Y, Adachi T, Miguchi M, Niitsu H, Sasada T, Shimomura M, Egi H, Oka S, Tanaka S, Chayama K, Sentani K, Oue N, Yasui W, Ohdan H.
-
Journal Title
J Gastroenterol.
Volume: 51
Pages: 447-457
DOI
Peer Reviewed
-
[Journal Article] TSPAN8, identified by Escherichia coli ampicillin secretion trap, is associated with cell growth and invasion in gastric cancer.2016
Author(s)
Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Naito Y, Oo HZ, Yasui W.
-
Journal Title
Gastric Cancer.
Volume: 19
Pages: 370-380
DOI
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer.2016
Author(s)
Oo HZ, Sentani K, Mukai S, Hattori T, Shinmei S, Goto K, Sakamoto N, Naito Y, Anami K, Trang PT, Yanagihara K, Oue N, Yasui W.
-
Journal Title
Gastric Cancer.
Volume: 19
Pages: 443-452
DOI
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] A case of tubulocystic carcinoma of the kidney with aggressive features.2016
Author(s)
Maeda Y, Goto K, Honda Y, Kuroda N, Sentani K, Yasui W, Hayashi T, Teishima J, Matsubara A, Nakamura Y, Toyota N, Iida M, Awai K.
-
Journal Title
Jpn J Radiol.
Volume: 34
Pages: 307-311
DOI
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] ALKR1275Q perturbs extracellular matrix, enhances cell invasion and leads to the development of neuroblastoma in cooperation with MYCN.2016
Author(s)
Ueda T, Nakata Y, Yamasaki N, Oda H, Sentani K, Kanai A, Onishi N, Ikeda K, Sera Y, Honda ZI, Tanaka K, Sata M, Ogawa S, Yasui W, Saya H, Takita J, Honda H.
-
Journal Title
Oncogene.
Volume: In press
Pages: In press
DOI
Peer Reviewed
-
-
-
-
[Journal Article] Characteristic miR-24 Expression in Gastric Cancers among Atomic Bomb Survivors.2015
Author(s)
Naito Y, Oue N, Pham TT, Yamamoto M, Fujihara M, Ishida T, Mukai S, Sentani K, Sakamoto N, Hida E, Sasaki H, Yasui W.
-
Journal Title
Pathobiology.
Volume: 82
Pages: 68-75
DOI
Peer Reviewed
-
[Journal Article] New molecular staging with G-factor supplements TNM classification in gastric cancer: a multicenter collaborative research by the Japan Society for Gastroenterological Carcinogenesis G-Project committee.2015
Author(s)
Sawada T, Yashiro M, Sentani K, Oue N, Yasui W, Miyazaki K, Kai K, Fushida S, Fujimura T, Ohira M, Kakeji Y, Natsugoe S, Shirabe K, Nomura S, Shimada Y, Tomita N, Hirakawa K, Maehara Y.
-
Journal Title
Gastric Cancer.
Volume: 18
Pages: 119-128
DOI
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] The Potential Role of Inflammation Associated with Interaction between Osteopontin and CD44 in a Case of Pulmonary Tumor Thrombotic Microangiopathy Caused by Breast Cancer.2015
Author(s)
Higashi A, Dohi Y, Uraoka N, Sentani K, Uga S, Kinoshita H, Sada Y, Kitagawa T, Hidaka T, Kurisu S, Yamamoto H, Yasui W, Kihara Y.
-
Journal Title
Intern Med.
Volume: 54
Pages: 2877-2880
DOI
Peer Reviewed
-
-
[Journal Article] Chlorinated Water Modulates the Development of Colorectal Tumors with Chromosomal Instability and Gut Microbiota in Apc-Deficient Mice.2015
Author(s)
Sasada T, Hinoi T, Saito Y, Adachi T, Takakura Y, Kawaguchi Y, Sotomaru Y, Sentani K, Oue N, Yasui W, Ohdan H.
-
Journal Title
PLoS One.
Volume: 10
Pages: e0132435
DOI
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Tubulosystic Carcinoma in the Left Kidney with Lymph Node Metastasis : Case Report.2015
Author(s)
Maeda Y, Honda Y, Higaki T, Iida M, Date S, Goto K, Sentani K, Yasui W, Kitano H, Kajiwara M, Matsubara A, Tsuji S, Kuroda N, Matsuura N, Toyota N, Awai
Organizer
第29回日本腹部放射線学会
Place of Presentation
浜松
Year and Date
2015-06-19 – 2015-06-20
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
