Diagnostics development of hepatocellular carcinoma by ultrahigh precision fluorescent imaging of tyrosine kinase receptor

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460450
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tohoku University
• Principal Investigator: Hara Yasuyuki 東北大学, 大学病院, 助教 (50636008)
• Co-Investigator(Kenkyū-buntansha): OHUCHI Noriaki 東北大学, 医学系研究科, 教授 (90203710) ; KAWAGISHI Naoki 東北大学, 大学病院, 准教授 (00333807) ; UMEDA Mika (渡辺 みか) 東北大学, 大学病院, 准教授 (20292344) ; GONDA Kousuke 東北大学, 医学系研究科, 教授 (80375435) ; TAKEDA Ikuo 東北大学, 医学系研究科, 大学院非常勤講師 (90420033) ; SEKIGUCHI Satoru 東北大学, 医学系研究科, 大学院非常勤講師 (20312580)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 肝細胞がん / 分子標的薬 / 蛍光ナノ粒子 / イメージング / 定量性

Outline of Final Research Achievements

In this study, we aim to develop the new method to measure the level of expression of sorafenib-targeting proteins by fluorescent nanoparticle quantitatively with a high degree of accuracy. We combined pegylated fluorescent nanoparticle with sorafenib via linker, and confirmed by infrared spectroscopy that synthesized nanoparticle had predictable structure. From the results of experiments using cultured cells, bond between sorafenib and fluorescent nanoparticle did not decrease the inhibitory capacity of cell proliferation, which sorafenib had originally. This showed that combination via linker did not decrease EFGR-binding capacity of sorafenib. In pathological analyses using paraffin-embedded cells, it was difficult to adjust photographing conditions, which was left open.

Free Research Field

肝臓外科