Development of therapeutic strategy by using recombinant-inbred model mouse strains of collagen disease

2015 Fiscal Year Final Research Report

• Project/Area Number: 25460496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Gifu University
• Principal Investigator: Miyazaki Tatsuhiko 岐阜大学, 医学部附属病院, 准教授 (80239384)
• Co-Investigator(Renkei-kenkyūsha): MASUMOTO Junya 愛媛大学, プロテオ医学研究センター, 教授 (20334914) ; NOSE Masato 愛媛大学, 医学系研究科, 名誉教授 (70030913) ; HASEGAWA Hitoshi 愛媛大学, 医学系研究科, 特任教授 (40164826) ; FUJINO Takahiro 愛媛大学, 学術支援センター, 准教授 (40292312)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 炎症 / 膠原病脂環モデルマウス / 組換え近交系 / 蛋白アナログ

Outline of Final Research Achievements

We have developed the polygene model murine strains of collagen diseases MXH/lpr. By using these strains, we aimed to evolve a new therapeutic strategy employing amino-acid analogue which might block the functional binding site of Opn AND/OR might work as a decoy of autoantigens. First, we established 23 strains of MXH/lpr recombinant inbred mice (among created 106 strains). Next, we screened the epitope of autoantigen of glomerulonephritis. Modulation of immune function by protein analogue administration was analyzed. Regarding the new drug delivery system, preliminary study of a novel DDS by Span 80 nano-vesicles were executed. Finally, the administration of amino-acid analogues to the collagen model mice strains were carried out.

Free Research Field

医歯薬学