2015 Fiscal Year Final Research Report
Induction of ex vivo-pulsed self dendritic cell vaccine against congenital toxoplasmosis
| Project/Area Number |
25460512
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Shinshu University (2014-2015)
Chiba University (2013) |
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Principal Investigator |
AOSAI Fumie 信州大学, 医学部, 委嘱講師 (80150316)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 先天性トキソプラズマ症 / トキソプラズマHSP70 / 樹状細胞ワクチン / 細胞傷害性T細胞(CTL) / T細胞ペプチドエピトープ |
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| Outline of Final Research Achievements |
１．Toxoplasma gondii infection during pregnancy induced the intrauterine growth retardation in fetus. The influences of T. gondii infection on placenta and fetal liver were shown by analyzing their dendritic cell (DC) maturation and Th polarization.
2. Not only CD8+ but CD4+ cytotoxic T lymphocytes (CTL) specific for T. gondii-infected cells were induced by the vaccination with a virulent tachyzoite-specific HSP70 (T.g.HSP70) gene targeting peripheral DC. Adoptive transfer experiments revealed that the vaccine-induced CTL possessed protective roles for toxoplasmosis at both acute and chronic phases of infection. CTL peptide epitopes of T.g.HSP70 which bound to murine and human MHC molecules were analyzed.
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| Free Research Field |
寄生虫学 感染免疫学
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