2015 Fiscal Year Final Research Report
Metabolomic profile of hepatitis C virus-infected hepatocytes
| Project/Area Number |
25460974
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHO Li 山形大学, 医学(系)研究科, 助教 (80344787)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | HCV / メタボローム / 細胞内代謝 / 感染 |
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| Outline of Final Research Achievements |
Hepatitis C virus (HCV) infection causes dramatic changes within infected hepatocytes. The aim of this study was to analyze the effect HCV infection has on the hepatocyte metabolome between HCVcc-infected and uninfected Huh7.5.1 cells in vitro using mass spectrometer for metabolomic analysis. More than 500 metabolites were identified and quantified by comparison of the uninfected hepatocytes. In total, 63 metabolites were siginificantly increased, 3 (citrate, cis-aconitate and iso-citrate) decreased relative to control. Based on the findings of this study, it would appear that many different metabolomics pathways are disrupted as a result of HCV infection, most notably glycolysis and TCA cycle metabolism. These results highlight a number of previously unreported metabolic interactions and give a more in depth into the effect that HCV has on host cell biochemical processes.
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| Free Research Field |
消化器内科
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