2015 Fiscal Year Final Research Report
The molecular mechanism of blood cell-cell interaction forming niche inducing CD34+ cell expansion and differentiation to endothelial progenitor cells in vascular regeneration
| Project/Area Number |
25461091
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cardiovascular medicine
|
|---|
| Research Institution | Tokai University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ASAHARA Takayuki 東海大学, 医学部, 教授 (20246200)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 血管内皮前駆細胞 / CD34 / Notchシステム / 血管再生 / 単球 / 血液細胞間ニッチ |
|---|
| Outline of Final Research Achievements |
We investigated blood cell-cell interactions to promote the expansion and differentiation of endothelial progenitor cells (EPCs) derived from CD34+ cells using the novel culture system developed by our laboratory. Peripheral blood mononuclear cells are divided into CD34+ stem cells, and CD34- cells of T lymphocytes (CD3+ cells), and monocytes (CD14+ cells). We found the promoting effect of CD34- cell populations on vascular regeneration capability of CD34+ cells via expansion and differentiation to endothelial progenitor cells (EPCs). Further, CD14+ cells turned out to be a key cell population responsible for the effect through a direct cell-cell interaction of Notch ligand/receptor system, Delta like -1 expressed on CD14+ cells to Notch receptor-1, or 2 on CD34+ cells, but not CD3+ cells.
The results indicate that CD34+ cells together with CD14+ cells form the functional ‘niche’ playing a critical role in vascular regeneration, providing a novel information in vascular biology.
|
| Free Research Field |
血管再生
|
