2015 Fiscal Year Final Research Report
Identification of the mechanisms for hereditary arrhythmic disease using human iPS cell technology
| Project/Area Number |
25461117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 心臓 / 再生医療 / 不整脈 |
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| Outline of Final Research Achievements |
We have previously reported that human induced pluripotent stem (iPS) cell technology is useful for disease specific in vivo cell model and drug screening. Since many kinds of ion channels express in the cardiomyocytes, it is desirable to investigate the effects of anti-arrhythmic drugs on electrophysiological properties in native cardiomyocytes.
In this study, first, we have succeeded in the induction of human iPS cell derived cardiomyocytes. QT PCR analyses revealed the expression of several ion channels such as SCN5A、CACNA1G、HCN4 in undifferentiated human iPS cells. Second, we investigated the effects of Bepridil on ion channel expression in human iPS cell derived cardiomyocytes. Bepridil dose dependently increased the expression of SCN5A, CACNA1, CACNA1G, HCN4. These results indicated that Bepridil may modulate ion channel expression in cardiomyocytes, resulting in the chronic effects of Bepridil in addition to the acute direct effect on the ion channels.
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| Free Research Field |
循環器
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