Development of a novel diagnostic system using next-generation sequencing for targetable gene alterations in lung cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: National Cancer Center Japan
• Principal Investigator: MATSUMOTO SHINGO 国立研究開発法人国立がん研究センター, 先端医療開発センター, 医員 (10392341)
• Co-Investigator(Renkei-kenkyūsha): TSUCHIHARA KATSUYA 独立行政法人国立がん研究センター, 先端医療開発センター, 分野長 (00415514) ; OHE YUICHIRO 独立行政法人国立がん研究センター, 中央病院, 副院長 (20233041) ; GOTO KOICHI 独立行政法人国立がん研究センター, 東病院, 科長 (90435719) ; KOHNO TAKASHI 独立行政法人国立がん研究センター, 研究所, 分野長 (80280783) ; KATOH MAMORU 独立行政法人国立がん研究センター, 研究所, 研究員 (40391916)
• Research Collaborator: SUGIYAMA ERI 独立行政法人国立がん研究センター, 先端医療開発センター, 外来研究員 (50775385)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 肺がん / マルチプレックス遺伝子診断 / 次世代シークエンス / NGS診断 / 融合遺伝子

Outline of Final Research Achievements

Establishment of a multiplex gene diagnostics is necessary for the development of precision medicine in lung cancer. We have generated a novel multiplex gene diagnostics by next-generation sequencing technology with a target enrichment system capturing genomic DNA. A variety of targetable gene alterations such as gene mutations and fusions occurring in lung cancer were successfully detected by our sequencing system. The detection sensitivity of targetable gene alterations by our diagnostic method was revealed to be more than 5% of cancer-derived genomic DNA. Further examination and evaluation for the clinical validity and utility are needed to establish this diagnostics. Additionally, the application of this method to gene diagnoses with peripheral blood samples; i.e., liquid biopsy, is an important issue to be essentially addressed.

Free Research Field

がんゲノミクス