2015 Fiscal Year Final Research Report
Roles of new cytokine IL-38 in inflammatory diseases
| Project/Area Number |
25461202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 炎症 / サイトカイン |
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| Outline of Final Research Achievements |
We generated anti-human IL-38 monoclonal antibodies in order to perform immunohistochemical staining and an enzyme-linked immunosorbent assay. While human recombinant IL-38 protein was not cleaved by recombinant caspase-1, chymase, and PR3 in vitro, IL-38 cDNA-overexpression produced a soluble form of IL-38 protein. Furthermore, immunohistochemical analysis showed that synovial tissues obtained from RA patients strongly expressed IL-38 protein. To investigate the biological role of IL-38, C57BL/6 IL-38 gene deficient (-/-) mice were used in an autoantibody-induced rheumatoid arthritis (RA) mouse model. In IL-38 (-/-) mouse, we observed a worse disease severity that was accompanied by higher IL-1beta and IL-6 gene expression in the joints as compared to control mice. Therefore, IL-38 acts as an inhibitor of the pathogenesis of autoantibody-induced arthritis in mice and may have a role in the development or progression of human RA (Biochemistry and Biophysics Reports 2015).
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| Free Research Field |
呼吸器内科
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