Establishment of a specific differentiation induction method to the kidneys constituent cells using a cell memory of the human kidney-derived iPS cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: The University of Tokyo
• Principal Investigator: TAKASE OSAMU 東京大学, 医学部附属病院, 講師 (60265684)
• Co-Investigator(Kenkyū-buntansha): HISHIKAWA Keiichi 東京大学, 医学部附属病院, 准教授 (50255460)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Toshiro 東京大学, 先端科学技術研究センター, 教授 (10114125) ; NAKAUCHI Hiromitsu 東京大学, 医科学研究所, 教授 (40175485)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: Epigenetic Memory / 腎臓由来iPS細胞 / 腎系統特異的分化誘導法 / 包括的メチル化解析 / 網羅的マイクロアレイ解析 / 新規腎臓発生・再生因子

Outline of Final Research Achievements

In this study, we have investigated the difference between fibroblast-derived iPS cells and the kidney-derived iPS cells by two kind renal lineage-specific differentiation induction methods. Both kidney-derived iPS cells by two induction methods became clear that easy to differentiate into kidney system. Also by comprehensive methylation analysis of each iPS cell, as a new renal lineage differentiation induction-related gene, hypermethylation group of 56 genes, and hypomethylation group of 17 genes were identified. In additional data of the comprehensive microarray analysis, it was narrowed down them to 8 genes. These genes are expected to be a kidney development and regeneration factor. We hope that it is useful in the future of the various studies.

Free Research Field

再生医学