Gene expression profiling of glomerular component cells to understand molecular mechanisms of progression of chronic kidney diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kyoto University
• Principal Investigator: Tsuchida Junichi 京都大学, 医学(系)研究科(研究院), 研究員 (10643570)
• Co-Investigator(Kenkyū-buntansha): MORI Kiyoshi 京都大学大学院医学研究科, メディカルイノベーションセンター, 特定准教授 (60343232)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 糸球体 / セルソーター / ポドサイト / 糖尿病性腎症 / 網羅的解析 / メサンギウム細胞 / 内皮細胞

Outline of Final Research Achievements

Although approximately 65% of new patients entering dialysis in Japan are diabetic nephropathy and chronic glomerular nephritis, there are few therapeutic medication. To elucidate the mechanisms of progression of chronic kidney disease, ｗe generate transgenic mice expressing glomerular targeted fluorescent protein. We identified the secreted protein A, which is expressed specifically in podocytes, by gene expression profiling of the diabetic mice. Its expression was reduced in diabetic nephropathy, and it inhibited the proliferation of cultured mesangial cells.

Free Research Field

腎臓内科学