Elucidation of restoration mechanisms in Alzheimer diseases by mobilized stem cells through activation of adipose tissue

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461331
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Chiba University
• Principal Investigator: KURODA Masayuki 千葉大学, 医学部附属病院, 特任准教授 (00253005)
• Co-Investigator(Kenkyū-buntansha): KUBOTA Yoshitaka 千葉大学, 医学部附属病院, 講師 (10375735)
• Co-Investigator(Renkei-kenkyūsha): BUJO Hideaki 東邦大学, 医療センター佐倉病院, 教授 (80291300)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脂肪幹細胞 / 脂肪組織 / アルツハイマー認知症 / ネプリライシン

Outline of Final Research Achievements

To elucidate self-defensive mechanisms against amyloid β protein (Aβ) in adipose-tissue, cellular responses of adipose-derived stem cells (ASC) and progenitor cells (ccdPA) to Aβ were examined. Proliferation of ASC was enhanced and migration-related gene expression in ASC was increased upon low dose Aβ exposure. The gene expression was increased by NEP gene knock-down in ASC, but not in ccdPA, and decreased by NEP overexpression in ASC. NEP knock-down resulted in reduced lipid-droplet accumulation upon adipogenic stimulation in both cells.
These data indicated that ASC and ccdPA respond differently to Aβ, and suggested that adipose tissue regulates self-defensive mechanisms against Aβ through NEP-mediated signaling.

Free Research Field

遺伝子細胞治療