Development of cartilage cells induction method and the establishment of the system to evaluate the effect of novel therapeutic drugs on skeletal dysplasia by using iPS cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461384
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: Miura Masako 京都大学, 医学(系)研究科(研究院), 講師 (80437235)
• Co-Investigator(Renkei-kenkyūsha): YASODA Akihiro 京都大学, 医学研究科, 講師 (50378642)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 内分泌学 / CNP / 骨代謝 / 低身長 / iPS細胞

Outline of Final Research Achievements

In this study, we have tried to establish the system to evaluate the effect of novel therapeutic drugs on skeletal dysplasia, such as achondroplasia, by using iPS cells. Achondroplasia chondrocyte proliferation, differentiation and matrix production is suppressed by the constitutive activation of FGFR3. Then, we performed the cartilage cells induction method to achondroplasia-derived iPS cells. In the process, we did assessment of the effectiveness of CNP by adding CNP. Comparing the CNP added group and the non-addition group showed a certain efficacy in the expression of genes associated with cartilage differentiation. We will keep trying to investigate the effects of CNP on the iPS cells derived from achondroplasia patient.

Free Research Field

医歯薬学