Dendritic cell differentiation from myeloid neoplasm

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: Mizuki Masao 大阪大学, 医学部附属病院, 准教授 (80283761)
• Co-Investigator(Kenkyū-buntansha): KANAKURA Yuzuru 大阪大学, 医学系研究科, 教授 (20177489) ; SHIBAYAMA Hirohiko 大阪大学, 医学系研究科, 講師 (60346202) ; FUJITA Jiro 大阪大学, 医学系研究科, 助教 (90608720)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 樹状細胞 / 白血病関連遺伝子 / cDC / pDC

Outline of Final Research Achievements

We have established the dendritic cell (DC) differentiation system from murine hematopoietic stem cell fraction. We have shown that type I leukemia-related gene abnormalities except for FLT3 and ras, blocked DC differentiation and preferentially induced the differentiation of conventional DC (cDC) compared to plasmacytoid DC. In patients with TEL-PDGFRbeta positive chronic myelomonocytic leukemia, cDC subset was found to be derived from leukemic clone. In patients with acute myelogeneous leukemia showing spontaneous DC differentiation, there was a CD8+T cell subset, which reacted with leukemia-specific antigen, WT1, suggesting that leukemia-derived DC could present leukemia-specific antigens. Finally, origin-unknown soft tissue tumor was found to be histiocytic lineage, by whole exome sequensing which showed MAP2K1 mutation.

Free Research Field

造血器腫瘍学