2015 Fiscal Year Final Research Report
Microvesicle-mediated interaction between multiple myeloma cells and bone marrow stroma
| Project/Area Number |
25461434
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 多発性骨髄腫 / 接着耐性 / 間質細胞 / Microvesicle / microRNA |
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| Outline of Final Research Achievements |
Multiple myeloma cells acquire the resistance to anti-cancer drugs via interaction with bone marrow stroma (cell adhesion-mediated drug resistance). In this study, we investigated the role of circulating microvescles (cMV) produced by myeloma cells in cell adhesion-mediated drug resistance. We isolated cMV from the co-culcture supernatant of myeloma cells and human bone marrow-derived stromal cells.We then extracted total RNA form cMV and subjected them to microRNA arrays, and found that myeloma-derived cMV was very rich in miR-155 and mir-135b. Functional studies revealed that the expression level of miR-155 was positively correlated with the sensitivity of myeloma cells to proteasome inhibitors. We are currently investigating the precise mechanisms by which miR-155 determines the sensitivity of myeloma cells to proteasome inhibitors.
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| Free Research Field |
血液腫瘍学・分子生物学
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