Approach to regulation of an anti-DNA antibody-knock-in mouse nephritis model

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: Kyoto University
• Principal Investigator: Yoshifuji Hajime 京都大学, 医学(系)研究科(研究院), 助教 (20422975)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膠原病学

Outline of Final Research Achievements

We established a systemic lupus erythematosus nephropathy model, anti-DNA antibody gene (R4A)-knock-in mice. To pursuit autoreactive B cells, we mated normal mice and R4A-knockin mice and obtained WT(a)/R4A(b)-F1 mice, in which we can identify normal B cells by anti-IgM(a) and anti-DNA-antibody-producing B cells by anti-IgM(b) antibodies. As a result, IgM(b)-positive cells were markedly decreased probably by a clonal deletion. RAG genes were markedly upregulated in IgM(b)-positive cells, implying that editing of immunoglobulin light chain was activated. These results elucidated a part of mechanisms how autoreactive B cells were deleted or rescued.

Free Research Field

医歯薬学