2015 Fiscal Year Final Research Report
Analysis of epigenome abnormality in Behcet's disease.
| Project/Area Number |
25461482
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Collagenous pathology/Allergology
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 末梢血有核細胞 / エピジェネティクス / ヒストンメチル化 / 活性化マーカー |
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| Outline of Final Research Achievements |
We analyzed peripheral blood nucleated cell subsets and histone modification by flow cytometry and transcriptome assay on the patients with Behcet's disease (BD). H3K27me3 and H3K4me3 were detected in CD4+ T cells, CD8+ T cells, γδ T cells, and CD16+CD66b+ neutrophils. H3K27me3 MFI levels were not significantly different in those cell types in BD. In contrast, H3K4me3 MFI levels of BD in γδ T cells were significantly increased compared with HC. H3K4me3/H3K27me3 MFI ratio was significantly lower in neutrophils of BD and higher in γδ T cells of BD than that of HC. H3K4me3 MFI and H3K4me3/H3K27me3 MFI ratio of active BD were significantly increased in γδ T cells as compared to inactives. The transciptome assay is still on going. Aberrant histone methylation may be associated with the pathogenesis of BD. It is suggested that histone methylation could be a new candidate-biomarker for BD.
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| Free Research Field |
リウマチ膠原病科
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