2015 Fiscal Year Final Research Report
High-sensitive MRD detecting method for leukemia using nano particles with sugar chain which can bind WT1 protein
| Project/Area Number |
25461601
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Yoshifumi 鹿児島大学, 医歯学域医学系, 教授 (20260680)
SUDA Yasuo 鹿児島大学, 理工学域工学系, 教授 (70179282)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ナノ粒子 / MRD / 急性白血病 |
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| Outline of Final Research Achievements |
The aim of this study is to develop a new, simple, and highly sensitive method to detect minimal residual disease (MRD) in acute leukemia. Nano particles having sugar chain which can bind specifically WT1 protein released from leukemic cells were made. After condensing WT1 protein by nano particles, WT1 level was measured by ELISA. Nano MRD method is much simpler and cheaper compared to other methods detecting MRD including flowcytometory or PCR. Nano MRD method is about 50-times more sensitive than regular ELISA method. Nano MRD method should be useful to detect MRD in leukemia patient.
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| Free Research Field |
小児がん
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