Pathogenesis of insufficient adrenocortical response in premature fetal sheep suffered from intrauterine inflammation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Tohoku University
• Principal Investigator: Akiyama Shizuko 東北大学, 医学(系)研究科(研究院), 非常勤講師 (80466549)
• Co-Investigator(Kenkyū-buntansha): MATSUDA TADASHI 東北大学, 病院, 准教授 (50361100) ; SAITO MASATOSHI 東北大学, 病院, 講師 (00451584) ; WATANABE TATSUYA 東北大学, 病院, 非常勤講師 (70400380)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 胎児 / 早産児 / 成育限界 / 子宮内炎症 / 副腎皮質ホルモン / CRH負荷試験 / ACTH / バゾプレッシン

Outline of Final Research Achievements

We planed to clarify the effect of intrauterine inflammation on the cortisol secretion and its circulatory response in premature lamb because the fetal endocrine potential to adapt the postnatal environment might have been already deteriorated by the inflammatory stress before birth. In consequence, we could store the plasma samples for 45 times of the corticotrophin releasing hormone test. Unfortunately, however, commercial kits for immunoassay of sheep AVP, ACTH and cortisol could not be available domestically during the study period. Instead, we have been developing the method of peptides and steroid profiling analysis using the LC-MS/MS.

Free Research Field

胎児生理学