Role of mebrane transporters in drug resistance of pancreatic cancer cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461956
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Yokohama City University
• Principal Investigator: UENO Yasuharu 横浜市立大学, 医学部, 助教 (60375235)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 癌幹細胞 / 膵癌 / プロテオーム解析

Outline of Final Research Achievements

The prognosis of pancreatic cancer remains very poor, the mainly reason might be the prominent resistance to chemoradiotherapy. However, the mechanism of chemoradiotherapy resistance in pancreatic cancer has not been fully understood. We aim to investigate the relationship between pancreatic cancer stem cells and chemoradiotherapy resistance, and identify the specific biomarkers, which promote tumor survival in pancreatic cancer with chemoradiotherapy.
Firstly, we transplanted human pancreatic cancer tissue into mice to construct pancreatic cancer mice model. With gemcitabine treatment, we found that the population of pancreatic cancer stem cells was increasing. Moreover, we identified expression of multiple transporters were upregulated after gemcitabine treatment with proteomic analysis, and these upregulated transporters were mainly detected in CD133+CD44+ cancer cells. Our results suggest that these transporters may exhibit important roles in the chemoradiotherapy resistance.

Free Research Field

幹細胞生物学