Effects of HLA-G expression and the genetic variation on graft survival - a reexamination of prior conclusions -

2016 Fiscal Year Final Research Report

• Project/Area Number: 25461957
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Nara Medical University
• Principal Investigator: Ishitani Akiko 奈良県立医科大学, 医学部, 非常勤講師 (40112544)
• Co-Investigator(Renkei-kenkyūsha): ICHINOHE TATSUO 広島大学, 医学系研究科, 教授 (80314219) ; UEMOTO SHINJI 京都大学, 医学系研究科, 教授 (40252449) ; TAKAHARA SHIRO 大阪大学, 医学系研究科, 教授 (70179547) ; MORII TAKESHI 奈良県立医科大学, 医学部, 講師 (70264851) ; YOSHIDA KATSUNORI 奈良県立医科大学, 医学部, 教授 (50192422)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: HLA-G / 移植片生着 / 腎移植 / 肝移植 / 造血幹細胞移植

Outline of Final Research Achievements

HLA-G is expressed on placental trophoblasts, the border of mother and fetus, and known to protect fetus from the maternal immune system. It was hypothesized that the immune suppressive function of HLA-G may help engraftment outcome at transplantation. This hypothesis was supported by several papers reporting that HLA-G protein in patient plasma or on the graft were associated with the graft survival.
In this study, HLA-G expression on the peripheral mononuclear cells and in the plasma of patients before and after transplantation were tested. We developed an ELISA to detect very low concentration of HLA-G protein in plasma. Using the method, 167samples from kidney transplantation, 111 from lever transplantation and 52 from hematopoietic stem cell transplantation were tested the HLA-G expression. No detectable levels of HLA-G were found in any samples. From this data, we conclude that the association of HLA-G expression and graft survival in those reports should be reexamined.

Free Research Field

免疫遺伝学