Establishment of the new therapy based on inflammatory mediator and DNA methylation in colorectal cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: Oki Eiji 九州大学, 大学病院, 講師 (70380392)
• Co-Investigator(Kenkyū-buntansha): MORITA Masaru 独立行政法人国立病院機構(九州がんセンター), その他部局等, 消化器外科部長 (30294937) ; SAEKI Hiroshi 九州大学, 大学病院, 講師 (80325448) ; KITAO Hiroyuki 九州大学, 医学研究院, 准教授 (30368617) ; IKEDA Tetsuo 九州大学, 大学病院, 准教授 (60585701)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: DNAメチル化 / 炎症性メデイエーター / 大腸癌 / プロスタグランジン / 低侵襲手術

Outline of Final Research Achievements

Global DNA hypomethylation is associated with increased chromosomal instability and plays an important role in tumorigenesis. The methylation status of the long interspersed nuclear element-1 (LINE-1) element is a useful surrogate marker for global DNA methylation. Using resected tumor tissues and the corresponding normal colorectal cancer, bisulfite pyrosequencing analysis was performed to quantify the LINE-1 methylation levels. p53 mutations in exons two to ten were detected by polymerase chain reaction direct sequencing. Chromosomal instability was assessed by single nucleotide polymorphism array comparative genomic hybridization analysis. The LINE-1 methylation level of colorectal cancer was lower than matched normal mucosa. The LINE-1 methylation levels in colorectal cancer had a significant inverse association with the methylation in the MLH1.

Free Research Field

消化器外科