Inducation of iPS cell and direct reprogramming - pathophysiology of atrial fibrillation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Saitama Medical University
• Principal Investigator: Iguchi Atsushi 埼玉医科大学, 医学部, 教授 (90222851)
• Co-Investigator(Kenkyū-buntansha): Domoto Satoshi 埼玉医科大学, 医学部, 助教 (00649337) ; Sembonmatsu Takaaki 埼玉医科大学, 医学部, 教授 (70216563)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: iPS 細胞 / 心房細動 / メイズ手術 / 皮膚組織 / 左心耳組織 / 筋線維芽細胞 / 線維芽細胞 / 山中4因子

Outline of Final Research Achievements

Most of the fibroblast separated from atrium tissue provided by Maize operation from a patient with atrial fibrillating, is a myofibroblast. Myofibroblasts are mesenchymal cells found in the pathological heart resulting from acute inflammation and chronic inflammation. If dermal fibroblasts are compared to the cardiac fibroblast, these two cells are not little difference in terms of morphology and growth potential, but the iPS cells are not derived using myofibroplast cells. Myofibroplast has high expression of α-SMA and MyoD at the genetic level, but a particular gene which inhibit the induction of iPS was not identified. Several papers described that in the process of iPS induction, downregulation of mesenchymal gene occurs after four factors introduced, but we found that if using a fibroblast cell, there is no downregulation of mesenchymal gene.

Free Research Field

心臓外科学