2015 Fiscal Year Final Research Report
Molecular analysis of mutated isocitrate dehydrogenase and its clinical application
| Project/Area Number |
25462242
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Tohoku University |
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Principal Investigator |
Kato Yukinari 東北大学, 医学(系)研究科(研究院), 教授 (00571811)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | IDH / IDH1-R132H / glioma / モノクローナル抗体 / HMba-2 |
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| Outline of Final Research Achievements |
Isocitrate dehydrogenase 1/2 (IDH1/2) mutations have been reported in gliomas, and are remarkably specific to a single codon in the conserved and functionally important arginine 132 residue (R132) of IDH1 or arginine 172 residue (R172) of IDH2. To date, we have established several monoclonal antibodies (mAbs) specific for IDH1/2 mutations. Here, we established MsMab-1 mAb that reacts with IDH1-R132H, IDH1-R132S, IDH1-R132G, and IDH2-R172M, but not with wild type IDH1/2. MsMab-1 stained the IDH1-R132S or IDH1-R132G -expressing glioma cells in immunohistochemistry. MsMab-1 is expected to be useful for immunohistochemical determination of IDH1/2 mutation-bearing tumors.
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| Free Research Field |
腫瘍生物学
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