A search for regulators of global histone modification in malignant brain tumor

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kochi University
• Principal Investigator: YAWATA TOSHIO 高知大学, 教育研究部医療学系臨床医学部門, 助教 (40380323)
• Co-Investigator(Kenkyū-buntansha): UEBA TETSUYA 高知大学, 教育研究部医療学系臨床医学部門, 教授 (00314203) ; HIGASHI YOUICHIRO 高知大学, 教育研究部医療学系基礎医学部門, 助教 (80380062)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 悪性脳腫瘍 / 幹細胞 / 代謝

Outline of Final Research Achievements

CD146 was identified as a surface marker highly expressing in glioma stem cells. CD146 regulates cell growth of glioma stem cells and is highly expressed in G2/M phase and its knockdown increases the proportion of cells in G0/G1 phase. It has been revealed that mitochondrial oxidative phosphorylation is dominant in glioma stem cells in contrast to in differentiated glioma cells using mainly aerobic glycolysis. Knockdown of CD146 in glioma cells switched from aerobic glycolysis to mitochondrial oxidative phosphorylation. In addition, expression of CD146 affected histone deacetylase activity in glioma stem cells. These results suggest that CD146 expressed in glioma stem cells is related to the cell growth and glucose metabolism and may affect on HDAC activity involved in stemness and differentiation.

Free Research Field

脳腫瘍学