Development of a new lumbar spinal canal stenosis therapy to prevent the progression of yellow ligament thickening.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462301
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka City University
• Principal Investigator: Dohzono Sho 大阪市立大学, 大学院医学研究科, 登録医 (10618945)
• Co-Investigator(Kenkyū-buntansha): TERAI Hidetomi 大阪市立大学, 大学院医学研究科, 准教授 (20382046) ; TOYODA Hiromitsu 大阪市立大学, 大学院医学研究科, 講師 (50514238) ; NAKAMURA Hiroaki 大阪市立大学, 大学院医学研究科, 教授 (60227931)
• Research Collaborator: TSUKIYAMA Kuniaki
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 黄色靭帯肥厚 / 機械的ストレス / 腰部脊柱管狭窄 / ウサギ / PCR

Outline of Final Research Achievements

The aims of this research were ① to prove that yellow ligament thickening model in rabbit is useful, and ② to assess the changes in the thickened yellow ligament histologically . With regard to ①, L2/3 and L4/5 were fixed with plates and harvested 8 and 16 weeks after surgery. We found that range of motion at L3/4 increased. With regard to ②, we found that toluidine blue staining showed red-purple areas, which are known to represent metachromasia indicating cartilagespecific matrix, were prominently increased in the yellow ligament. Moreover, these cells and the matrix were positively stained with an antitype 2 collagen antibody by immunohistochemistry.

Free Research Field

整形外科