2016 Fiscal Year Final Research Report
The mechanisms of autophagic cell death induction by histone deacetylase inhibitor SAHA
| Project/Area Number |
25462338
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | ヒストン脱アセチル化酵素阻害剤 / オートファジー細胞死 / 多剤耐性骨肉腫細胞株 |
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| Outline of Final Research Achievements |
There was no correlation between the expression of autophagy-related protein and acetylation of the histone. I was able to confirm that quantity of population of G2/M increased by SAHA. However, there was no correlation between G2/M introduction time and LC3-2 expression increasing time. SAHA induced the formation of autophagosome in a cell. I observed the period of the formation of autophagosome , there was no tendency of this period. I tried the making of the multidrug-resistant osteosarcoma cell animal model using osteosarcoma cell line: Saos2, but this cell line died the concentration of 25 ng/ml Dox. We could not make the multidrug-resistant Saos2 cell line.
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| Free Research Field |
整形外科
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