Research for Molecular Target of Osteosarcoma treatment

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462342
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kagoshima University
• Principal Investigator: Setoguchi Takao 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (40423727)
• Co-Investigator(Kenkyū-buntansha): KOMIYA Setsuro 鹿児島大学, 医歯学域医学系, 教授 (30178371) ; NAGANO Satoshi 鹿児島大学, 医歯学域医学系, 准教授 (50373139)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨肉腫 / Hedgehog / GLI2 / RPS3 / metastasis

Outline of Final Research Achievements

We researched the molecular target for osteosarcoma treatment. We found that Hedgehog pathway is upregulated in human osteosarcoma. Inhibition of GLI family zinc finger 2 (GLI2) prevent osteosarcoma invasion. Invasion assay showed that combination of arsenic trioxide (ATO) and vismodegib (SMO inhibitor) prevented invasion of human osteosarcoma cells. Xenograft model showed that combination of ATO and vismodegib prevented lung metastasis of osteosarcoma. We found that expression of ribosomal protein S3 (RPS3) is regulated by GLI2. RPS3 promoted the invasion of osteosarcoma cells. These findings suggest that GLI2-RPS3 pathway might be a new therapeutic target for osteosarcoma.

Free Research Field

骨軟部肉腫