An exploratory study on a novel factor related to the decision of bone metastasis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462356
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: National Hospital Organization, Kyushu Cancer Center
• Principal Investigator: Takiguchi Soichi 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, 研究員 (00280793)
• Co-Investigator(Kenkyū-buntansha): IGUCHI Haruo 四国がんセンター, 臨床研究センター, 顧問 (90393438)
• Co-Investigator(Renkei-kenkyūsha): MATSUSUE Kimihiko 福岡大学, 薬学部, 准教授 (10389364)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨転移 / ケモカイン / 走化性 / 足場非依存性増殖

Outline of Final Research Achievements

To investigate the molecular mechanisms of lung cancer-induced bone metastasis, we established a bone-seeking subclone (HARA-B4) from a human squamous lung cancer cell line (HARA) using an in vivo selection method. From the comparison of comprehensive gene expression profiles between HARA and HARA-B4, we identified CXC chemokine ligand 14 (CXCL14) as a critical factor for the formation of bone metastasis. CXCL14 was expressed 5-fold greater in HARA-B4 than in HARA, and shown to be involved in the anchorage-independent growth of cancer cells, and enhancement of cancer cell tropism to the bone possibly by the recruitment of bone marrow cells to cancer cells. Furthermore, in clinical specimens of lung cancer-induced bone metastasis, expression of CXCL14 was observed in the tumor cells infiltrated in bone marrow in all specimens examined.

Free Research Field

分子生物学