Elucidation of the calcified promotion mechanism of osteoblasts by the carbon nanotubes

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462365
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Shinshu University
• Principal Investigator: HANIU Hisao 信州大学, 学術研究院医学系, 准教授 (30252050)
• Co-Investigator(Kenkyū-buntansha): USUI Yuki 信州大学, 医学部附属病院, 特任研究員 (00467169) ; TSUKAHARA Tamotsu 長崎大学, 医歯(薬)学総合研究科, 准教授 (00529943)
• Co-Investigator(Renkei-kenkyūsha): SAITO Naoto 信州大学, 学術研究院保健学系, 教授 (80283258)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨・軟骨代謝学 / バイオマテリアル / カーボンナノチューブ / 骨芽細胞

Outline of Final Research Achievements

We examined the relationship between Multi-walled carbon nanotubes (MWCNTs) and a MC3T3-E1 preosteoblast cell line. MWCNTs were combined with three different dispersants (FBS, gelatin, or CMC). Cell viability was measured under various culture conditions and morphological analysis was performed. We also assessed several osteoblast differentiation markers. Inhibition of cell proliferation was witnessed only under specific conditions, such as when MC3T3-E1 not cultured in calcification medium was exposed to MWCNTs dispersed in gelatin. Furthermore, cell proliferation increased with all dispersants when the cells were cultured in calcification medium. In morphological observations, whereas the cells displaying proliferation inhibition had internalized MWCNTs into the cytoplasm, the biomaterial was found to be adhered to the cell membrane in other conditions. MWCNTs up-regulated the expression of one marker of osteoblast differentiation, regardless of the dispersant.

Free Research Field

生体材料学