2015 Fiscal Year Final Research Report
Mechanism of articular cartilage repair and prevention of cartilage degeneration with tenascin C and application for treatment
Project/Area Number |
25462367
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Mie University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
SUDO Akihiro 三重大学, 大学院医学系研究科, 教授 (60196904)
YOSHIDA Toshimichi 三重大学, 大学院医学系研究科, 教授 (80166959)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 関節軟骨 / テネイシン |
Outline of Final Research Achievements |
We implanted Gellan-GS soaked in TNC into full-thickness osteochondral defects of the patellar groove of rabbits. Histologically as well as macroscopically, we found satisfactory repair of cartilage. Using mice, full-thickness osteochondral defects were created and the defects were filled with TNC. Histologic study showed satisfactory repair of cartilage. In posttraumatic osteoarthritis model with transection of anterior cruciate ligament and medial collateral ligament, we performed intra-articular injection of TNC. TNC administration markedly protected the articular cartilage from proteoglycan depletion for 6 weeks. These studies indicated that TNC could promote cartilage repair and protect cartilage degeneration in vivo. Although TNC upregulated the expression of ADAMTS4, TNC downregulated the expression of ADAMTS5.
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Free Research Field |
医歯薬学
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