Elucidation of the epigenetic regulatory network during cell differentiation of spermatogonial stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462541
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Azemoto Masataka 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70264736)
• Co-Investigator(Kenkyū-buntansha): KAMISAWA HIDEYUKI 名古屋市立大学, 大学院医学研究科, 研究員 (00551277) ; NISHIO HIDENORI 名古屋市立大学, 大学院医学研究科, 助教 (10621063) ; KOHRI KENJIRO 名古屋市立大学, 学長 (30122047) ; HAYASHI YUTARO 名古屋市立大学, 大学院医学研究科, 准教授 (40238134) ; KOJIMA YOSHIYUKI 福島県立医科大学, 医学部, 教授 (60305539)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 精子幹細胞 / エピゲノム

Outline of Final Research Achievements

Recently, epigenetic regulation has benn regarded as an important system of gene regulation. There are DNA methylation or histone modification as typical regulatory system, and it seems that epigenome is involved in the determination of cell fate. From the results of our previous work, we considered that the dysfunction of spermatogonial stem cells is one of the reasons why spermatogenesis is disrupted in cryptorchidism. In the present research, we aimed the specific epigenetic regulatory network during cell differentiation of spermatogonial stem cells. We assessed the spermatogonial stem cells in the testes of each developmental stages using experimental animals. As a result, we detected that Kdm5a, demethylase for histone H3K4, and miR-135a (microRNA) are involved in differentiation of spermatogonial stem cells.

Free Research Field

泌尿器科学