Inner cell mass division during human blastcyst formation in vitro culture system.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462549
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Akita University
• Principal Investigator: Terada Yukihiro 秋田大学, 医学(系)研究科(研究院), 教授 (10260431)
• Co-Investigator(Kenkyū-buntansha): KABASHIMA Katsuya 秋田大学, 医学系研究科, 技術系補佐員 (30615422) ; KUMAGAI Jin 秋田大学, 医学系研究科, 准教授 (60333936) ; KUMAZAWA Yukiyo 秋田大学, 医学系研究科, 助教 (70400504)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ヒト胚 / 胚発育 / 胚内部細胞塊分裂 / 胚凍結 / 胚盤胞

Outline of Final Research Achievements

This study is an examination about the unnatural separation of the inner cell mass cells during human in vitro blastocyst formation.　This phenomenon was found by continuous observation by timelapse cinematography in the in vitro culture system of the mammalian embryo. This behavior was frequently observed in embryos after the freeze thaw (JRD,2016), also frequently observed under condition with the functional inhibitor of the gap junction (JARG,2015). Furthermore, the embryo which this behavior was found was thought to have low the developmental potential. These findings suggested that human embryo freeze thaw have influence on embryonic development behavior and the quality of embryos. The new scientific indicator of the embryo evaluation will be developed by the continuous observation/evaluation of the embryonic development.

Free Research Field

産婦人科学