The mechanism of signal for regulating differentiation in human trophoblast stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462562
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto University
• Principal Investigator: TAKAO TOMOKA 京都大学, 医学(系)研究科(研究院), 研究員 (40612429)
• Co-Investigator(Kenkyū-buntansha): WAKE NORIO 九州大学, 環境発達医学研究センター, 特任教授 (60091584)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分化制御 / 胎盤 / 栄養膜幹細胞

Outline of Final Research Achievements

We analyzed the new gene for regulating the differentiation of human trophoblast lineage using HTR-8/SVneo cells. We isolated the 3 trophoblast lineages including each functions; human trophoblast stem/precursor(hTS) cells, syncytiotrophoblast(ST) and extravillous trophoblast(EVT)from HTR-8/SVneo. DNA microarray analysis showed that IFIT2(interferon induced protein with tetratricopeptide repeats 2) was up-regulated in ST cells or TRIB3（tribbles pseudokinase 3）was up-regulated in EVT cells. We transfected its into hTS cells. Upregulation of each gene expression was increased trophoblast differentiated cell markers such as HLAG or HCGB.

Free Research Field

分子生物学