A basic and clinical approach to risk factors of recurrent pregnancy loss – Identification of a novel antibody associated with seronegative obstetric antiphospholipid syndrome (SN-APS)

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462578
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: Takesshita Toshiyuki 日本医科大学, 大学院医学研究科, 大学院教授 (60188175)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 不育症 / 抗リン脂質抗体症候群（APS) / 血清学的陰性APS / 補体分子C9

Outline of Final Research Achievements

There is a subpopulation of patients with clinical symptoms of APS, but the lack of serological markers (seronegative APS). We collected peripheral blood from SN-APS outpatients with known thrombotic predisposition (protein S deficiency, protein C deficiency, factor XII deficiency, or presence of anti-phosphatidylethanolamine antibodies). Two-dimensional immunoblotting was performed with pooled control and SN-APS sera as the primary antibody, and complement molecule C9 were identified as reactive spots specific to SN-APS serum. Autoantibody for C9 was detected in 2/14 SN-APS patients with no known thrombotic predisposition, and in 1/12 SN-APS patients with a known thrombotic predisposition other than APS by WB using purified C9 treated with iodoacetamide. The reaction was not seen in control sera (0/4). This study suggests that some SN-APS pathologies relate to autoantibodies that react to specific C9 epitopes expressed under fixed conditions.

Free Research Field

産婦人科