The role of FGF signal for the maturation of stereocilia in hair cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462638
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kyoto University
• Principal Investigator: Kita Tomoko (嶋知子) 京都大学, 医学研究科, 特定助教 (20362519)
• Research Collaborator: Sabyasachi Rakshit Indian Institute of Science Education and Research, Assistant Professor
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 内耳有毛細胞 / 感覚毛 / FGFシグナル / 形成

Outline of Final Research Achievements

The aim of this project is to clarify the effect of FGF signal on the morphology of stereocilia and transductional channeln of hair cells. We've reported reported that FGF signal helps not only the ear formation in early embryo stage but also kinocilia formation. FGFR1 exists in the tip of stereocilia temporary (P2-P7 in cochlea organ and P1-P10 in vestibular organ) using lab-made antibody. This was confirmed using the purchased antibody which recognize same region. Sequencing of FGFR1 gene from mouse cochlea sample on that transient stage, we found the new isoform which doesn't contain neither transmembrane nor intracellular domain. Although we tried to produce FGFR1 conditional KO mice, the estimation of these mice because of the variability of KO ratio.

Free Research Field

耳科学