Novel treatment for tracheal stenosis by gene therapy and molecular target therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462699
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: National Defense Medical College
• Principal Investigator: Tomifuji Masayuki 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部医学科専門課程, 講師 (80327626)
• Co-Investigator(Kenkyū-buntansha): KOJI Araki 防衛医科大学校, その他部局, 准教授 (70317220) ; AKIHIRO Shiotani 防衛医科大学校, その他部局, 教授 (80215946)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: tracheal stenosis / laryngeal stenosis / sendai virus / c-myc / FIR

Outline of Final Research Achievements

Animal model of laryngeal and tracheal stenosis was established by brushing from tracheostoma. We chose Sendai virus vector (SeV) as a gene transfer method. Gene transfer to laryngotracheal wound was possible by spraying SeV in 3-4 % of wound area. Administration of FIR gene which is a suppressor of c-myc gene carried by SeV improved tracheal stenosis and survival rate. Immunohistochemical staining of c-Myc also showed low expression level by FIR gene administration in layngotracheal brushing model.
Effect of systemic administration of immunosuppressant tacrolimus for prevention of tracheal stenosis was evaluated. Low dose tacrolimus administration showed preventative effect of tracheal stenosis by suppressing calcineurin/ nuclear factor of activated T cell / interleukin 2 pathway.

Free Research Field

laryngology, otorhinolaryngology