Intestinal surfactant protein in the intestinal failure of low birth weight children

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462781
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Osaka University (2014-2015); Hyogo Medical University (2013)
• Principal Investigator: Okuyama Hiroomi 大阪大学, 医学(系)研究科(研究院), 教授 (30252670)
• Co-Investigator(Kenkyū-buntansha): Saka Ryuta 大阪大学, 大学院医学系研究科, 助教 (00459190) ; Kubo Syuji 兵庫医科大学, 医学部, 教授 (10441320) ; Sasaki Takashi 兵庫医科大学, 医学部, 講師 (20388573) ; Yanagihara Itaru 大阪府立母子保健総合医療センター, 研究所, 部長 (60314415)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: SP-D / 壊死性腸炎

Outline of Final Research Achievements

Surfactant protein (SP)-A and -D is member of collectin, which plays an important role in innate immunity. The function of SP-A and -D has been studied mainly in the airway. We confirmed the expression of SP-A and -D in the murine fetal intestines and pancreas from the latter of the pregnancy. In human beings, SP-A is localized to airway, so we studied the function of SP-D in the human intestine. Necrotizing enterocolitis (NEC) remains life-threatening disease in the premature infants. NEC may depend on the TLR-4 overexpression in the immature (premature) intestines. Platelet activating factor (PAF) induced TLR-4 overexpression in human intestinal cell line INT407, originally derived from human embryonal intestines. The TLR-4 agonist lipopolysaccharide (LPS) induced IL-8 elevation in TLR-4 overexpressed INT407. Recombinant SP-D (full-length) significantly attenuated the inflammation in the above model. SP-D may have a protective effect in the development of NEC in preterm infants.

Free Research Field

小児外科